The Effect of Albizia lebbeck on Impairment of Interlukin-2 (IL-2) Gene Expression

Abstract

The determine of incorporated impacts of an ethanolic Albizia lebbeck leaf extract and Killed Whole Cell Sonicated Ag-Klebsiella pneumoniae (KWCSAg-KP) on diminish of IL-2 gene expression. Five groups of eight rabbits each were used in this experiment. Subcutaneous injection of 1000 μg/ml KWCSAg-KP was given to the first Group, while the second received subcutaneous KWCSAg-KP vaccination (1000 μg/ml) and oral Albizia lebbeck extract (300 mg/kg). Oral Albizia lebbeck extract at a dosage of 150 mg/kg and 1000 μg/ml KWCSAg-KP were given to the third group. For the fourth group, oral dosage of Albizia lebbeck extract was only 300 mg/kg. The fifth group was given PBS to serve as a control. Assessments of interleukin-2 (IL-2) gene expression by Real time-quantitative polymerase chain reaction were used to evaluate the outcomes. The first, second and third groups revealed an increase in IL-2 gene expression after vaccination compared to the control group; in contrast the fourth group exhibited a decrease in IL-2 gene expression compared to the other groups throughout the study and the changes were statistically significant (P< 0.05) among groups. It was concluded that the Albizia lebbeck extraction has anti-inflammatory properties by impairs IL-2 gene expression.