Predominance of antibiotics resistance of Escherichia coli isolated from Urinary tract infection patients in diabetic and non- diabetic woman

Abstract

Escherichia coli is the commonest bacterial uropathogen of UTIs, the commonest infections in diabetic and non-diabetic patients. Better understanding of their main resistance mechanisms to commonly used antibacterial agents will help to reduce the burden of this infection. The prevalence of drug resistant uropathogenic E.coli isolates from  diabetic and non-diabetic patients attending health facilities. These patients were further categorized into the ‘diabetic group based on the following definitions: the diabetic group consisted of patients with prior diagnosis of DM (complicated or uncomplicated), with a blood HbA1C level above 6,5% . Patients with T2DM were selected in line with the standard definition of the American Diabetes Association.      This study aimed to define the antimicrobial Resistance of Escherichia coli isolated from diabetic and nan-diabetic from UTI patients in al.najaf , iraq. By assessing the susceptibility patterns of these bacterial strains, we can gain valuable insights into the burden of antibiotic resistance in this population. Such information can help guide the development of appropriate treatment strategies and inform public health policy decisions. In this study, 300 clinical indivisuals were collected urine samples to patients divided into groups (A and B), each containing 150 samples. all isolates were tested for antimicrobial sensitivity using the Kirby-Bauer disk diffusion method in accordance with the definition provided by the Committee of Clinical Laboratory International Standards (CLSI, 2020) on diagnostic sensitivity test plates. The McFarland standard was used to prepare bacterial inoculum suspensions for antimicrobial susceptibility testing. Mueller Hinton agar plates were streaked using a sterilized cotton swab. sterile  Antibiotics  were used. its were highly Resistance to most of the selected antibiotics. E.coli showed a high resistance to ampicillin, Both group A and group B demonstrated a high level of resistance to ampicillin 61(96.82%) vs 47(92.15%) respectively,Cefotaxime 59(93.65%) vs 45(88.23%),Ceftazidime 58(92.06%) vs 43(84.31%), Trimethoprim–sulfamethoxazol, 58(92.06%) vs 44(86.27%). also results showed that broad-spectrum antibiotics such as imipenem, meropenem and nitrofurantoin would be the first line and the most effective antibiotics for the empirical treatment of urinary tract pathogens due to their low resistance rates. its were 6(9.52%) vs 3(5.88 %),4(6.34%) vs 2(3.92 %) and 23(36.5%) vs 12(23.52%) respectively.