Effect of tRNALys DNA m.8360 A>G Human Mitochondria Mutation on Diabetes
Abstract
This study points out for the first time that mitochondria are energy production sites and other metabolic functions mitochondria DNA(mDNA) is only maternal inherited. Mutations in(mDNA) are associated with many human mitochondrial diseases linked with disorders in the oxidation production of energy. It can lead to diabetes. this study is designed to investigate the mitochondria tRNALys DNA m.8360 A>G mutation in Iraq families with type 2 diabetes mellitus using microgenealliment sequencing technique. Eleventh Iraqi families underwent the experiment.Genomic DNA was isolated from whole blood samples following a standard protocol and was measured by a Nanodrop device, where the purity of DNA was 1.8-1.9 at 260 nm and its concentration was 22.4ng. Eight mothers out of eleven (72.7%) have this mutation for this result it can concluded that the A8360G mtDNA mutation of the tRNALys and associated with maternal diabetes.
