Enhancement of Cyclosporine Loaded with Chitosan Nanoparticles on Some Hematologic Parameters of Benzene-Induced Aplastic Anemia in Adult Male Dogs.

Abstract

Aplastic anemia, characterized by a deficiency of hematopoietic stem cells, leads to pancytopenia in peripheral blood and bone marrow cells. The aims of this study to evaluate the therapeutic efficacy of cyclosporine alone, cyclosporine loaded with chitosan nanoparticles, and chitosan nanoparticles alone, with emphasing on restoring normal blood cell levels and preventing disease complications. Twenty five adult male dogs aged ‎from 12 to 24 month were randomly divided into five groups: the first group was control negative (C-), given only distilled water, and the positive control (C+), which was untreated. The first treatment group (20 mg/kg) treated with chitosan nanoparticles, the second treatment group was with cyclosporin (5 mg/kg) alone, and the third treatment group was with cyclosporin loaded with chitosan nanoparticles (chitosan nano particles 5mg + cyclosporine 20mg/kg for each one). After a 60-day treatmentcourse. Assessments of adult male canines included serum IL2 levels and a complete blood count.When compared to group C+, all treated groups exhibited a signifiantly improved in RBCs, Hct, Hb, and RDWc. The group treated with cyclosporine loaded with chitosan nanoparticles shown the strongest responseto treatment when compared to the other trea drop in IL2 levels, which tment groups, the cyclosporine group loaded with chitosan nanoparticles showed a considerable was in close agreement with the negative control. In conclusion, the research shows that cyclosporine loaded onto chitosan nanoparticles has the best therapeutic effect in treating benzene-induced aplastic anemia in adult male dogs. The treatment showed an improvement in all the factors tested, which indicating the nanomaterial role of the in delivering the treatment to the desired target.