Detection of Virulence Genes (iap) in Listeria Monocytogenes in Children and Pregnant Women in Baghdad’s Hospitals

Abstract

Studying listeriosis is important to find out what the disease does to pregnant women and newborns and how dangerous it is for them and virulence gene that important know what the affect of it. The gene responsible for the encoding part of Listeria monocytogenes EGD has been successfully isolated and its sequence has been determined. The gene was called IAP (invasion-associated protein) because of its potential to participate in invasion. The IAP gene encodes a polypeptide that has a total of 484 amino acids, including a 27-amino-acid signal peptide. This signal sequence triggers the production of a 47.5 kDa polypeptide that is considered to be mature. The analysis of the deduced amino acid sequence showed that p60 is a protein that possesses basic properties (the iselectric point of 9.3) and includes a domain that is composed of 19 Thr-Asn repeats. Both DNA-based hybridization tests and immunological analyses demonstrated the presence of genes associated with IAP and related proteins in all of the tested Listeria species (9, 9a, 33). PrfA lacks control over the IAP gene. Recently, it was demonstrated that the control of IAP expression is posttranscriptional, as evidenced by Wuenscher's team. in 1993.